Fetal Fraction Estimate in Twin Pregnancies Using Directed Cell-Free DNA Analysis
نویسندگان
چکیده
منابع مشابه
Fetal fraction estimate in twin pregnancies using directed cell-free DNA analysis.
OBJECTIVE To estimate fetal fraction (FF) in monozygotic and dizygotic twin pregnancies. METHODS Maternal plasma samples were obtained from 35 monochorionic twin pregnancies with male fetuses (monozygotic) and 35 dichorionic pregnancies discordant for fetal sex (dizygotic) at 11-13 weeks' gestation. Cell-free DNA was extracted and chromosome-selective sequencing with digital analysis of selec...
متن کاملCell-free fetal DNA levels in pregnancies conceived by IVF.
BACKGROUND Increased second-trimester levels of maternal serum HCG in IVF conceptions lead to an increased false-positive rate in Down syndrome screening. Increased levels of cell-free fetal DNA (cffDNA) in maternal plasma have been correlated with increased HCG levels. Our aim was to determine whether cffDNA levels are elevated in IVF pregnancies compared with natural pregnancies. METHODS Si...
متن کاملCell-free DNA analysis for trisomy risk assessment in first-trimester twin pregnancies.
OBJECTIVE To examine the clinical implementation of chromosome-selective sequencing of cell-free DNA (cfDNA) in maternal blood and an algorithm that relies on the lower fetal fraction contribution of the 2 fetuses in the assessment of risk for trisomies in twin pregnancies. METHODS Risk for trisomies 21, 18 and 13 by cfDNA testing were estimated in stored plasma samples obtained at 11-13 week...
متن کاملPerformance of screening for aneuploidies by cell-free DNA analysis of maternal blood in twin pregnancies.
OBJECTIVES To report clinical implementation of cell-free DNA (cfDNA) analysis of maternal blood in screening for trisomies 21, 18 and 13 in twin pregnancies and examine variables that could influence the failure rate of the test. METHODS cfDNA testing was performed in 515 twin pregnancies at 10-28 weeks' gestation. The failure rate of the test to provide results was compared with that in 184...
متن کاملAssessment of fetal sex chromosome aneuploidy using directed cell-free DNA analysis.
OBJECTIVE To examine the performance of chromosome-selective sequencing of cell-free (cf) DNA in maternal blood for assessment of fetal sex chromosome aneuploidies. METHODS This was a case-control study of 177 stored maternal plasma samples, obtained before fetal karyotyping at 11-13 weeks of gestation, from 59 singleton pregnancies with fetal sex chromosome aneuploidies (45,X, n = 49; 47,XXX...
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ژورنال
عنوان ژورنال: Fetal Diagnosis and Therapy
سال: 2013
ISSN: 1015-3837,1421-9964
DOI: 10.1159/000355653